Infection of Gammarus duebeni populations by two vertically transmitted microsporidia; parasite detection and discrimination by PCR–RFLP

We screened a population of the brackish water crustacean Gammarus duebeni from the Isle of Cumbrae for the presence of vertically transmitted microsporidia. We compared 2 screening techniques; light microscopy and PCR-based detection using generic 16S rDNA microsporidian primers. Fifty percent of females from this population tested positive for vertically transmitted microsporidia. The PCR screen was 100% efficient in comparison with existing LM based screening. In addition, the PCR screen produced bands of 2 sizes suggesting that more than 1 species of microsporidian was present. Sequencing revealed 2 distinct species of vertically transmitted microsporidia; 33% of females were infected with the feminizer Nosema granulosis and 17% were infected with a new species which we provisionally designate Microsporidium sp. On the basis of sequence information, we developed a discriminatory PCR–RFLP test based on MspI and HaeIII digests. This screen allows rapid detection and discrimination of vertically transmitted microsporidia in natural field populations. We applied the PCR–RFLP screen to a second G. duebeni population from the Isle of Man. This population also hosted these 2 parasite species. In total 45% of females harboured N. granulosis and 10% harboured Microsporidium sp. No dual-infected individuals were found in either population. The occurrence of 2 vertically transmitted parasites within a population has implications for our understanding of parasite–host relationships in the field and we discuss factors affecting the dynamics of parasite–parasite competition and coexistence

The modest professor:Interpretive charity and interpretive humility in John Rawls's lectures on the history of political philosophy

Governmen

An investigation into wound infection and abscess formation in a mine

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Elastic and Mechanical Properties of Human Dentin

Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/67340/2/10.1177_00220345580370041801.pd

Strength Properties of Waxes at Various Temperatures and Their Practical Application

Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/66922/2/10.1177_00220345670460013101.pd

On the chirality of quark modes

A model for the QCD vacuum based on a domainlike structured background gluon field with definite duality attributed to the domains has been shown elsewhere to give confinement of static quarks, a reasonable value for the topological susceptibility and indications that chiral symmetry is spontaneously broken. In this paper we study in detail the eigenvalue problem for the Dirac operator in such a gluon mean field. A study of the local chirality parameter shows that the lowest nonzero eigenmodes possess a definite mean chirality correlated with the duality of a given domain. A probability distribution of the local chirality qualitatively reproduces histograms seen in lattice simulations.Comment: RevTeX4, 5 figures, 14 page

Class II Furcations Treated by Guided Tissue Regeneration in Humans: Case Reports

Peer Reviewedhttps://deepblue.lib.umich.edu/bitstream/2027.42/142299/1/jper0510.pd

Risk homeostasis theory - A study of intrinsic compensation

Risk homeostasis theory (RHT) suggests that changes made to the intrinsic risk of environments are negated in one of three ways: behavioural adjustments within the environment, mode migration, and avoidance of the physical risk. To date, this three-way model of RHT has little empirical support, whilst research findings on RHT have at times been diametrically opposed. A reconciliation of apparently opposing findings might be possible by suggesting that extrinsic compensation fails to restore previously existing levels of actual risk in cases where behavioural adjustments within the environment are incapable of negating intrinsic risk changes. This paper reports a study in which behavioural adjustments within the physical risk-taking environment are capable of reconciling target with actual risk. The results provide positive support for RHT in the form of overcompensation for the intrinsic risk change on specific driver behaviours